J. Physiol. Biochem., 57 (4), 343-344, 2001 Verapamil sensitisation to alkaloids on colchicine-selected human colon adenocarcinoma cells
exposed to different drug doses for 1 hour
(MDR), the main cause of failure in cancer
until colony formation. The effect of ver-
extrusion of the antineoplastic drugs from
apamil as a drug sensitiser was performed
by the exposure of colon cells to different
that acts as an extracting pump (2). There-
multidrug resistance associated with P-gly
used to assess the normal distribution of
the data. Additional statistical analyses
caused by this protein. Verapamil reverses
were made with the student’s t-test. A sig-
drug resistance by competitively inhibit-
ing the binding of P-gly and the cytotoxic
drugs, allowing for the drug to accumulate
on the sensitisation of the selected cell
The aim of this study is to investigate if
loids. In all cases, the surviving fraction
a multidrug resistant colon carcinoma cell
line that has been selected with colchicine
added together with the drug. The highest
for verapamil increased sensitivity to 0.5
selection. Verapamil increased sensitivity
2/1cch), selected from the parental line (6)
drugs. The present results are similar to
findings (3) reporting that verapamil (1-5
6RADR cell lines. While other authors (4)
10 µg/ml of verapamil in KB-C1 cells.
PIRKER et al. (7) have demonstrated that 5
antineoplastic drug activity and verapamil
µg/ml verapamil is sufficient to complete-
ly reverse the resistance to CCH in these
exponentially growing culture in drug free
cells. Therefore, it appears that a vera-
cient to produce a significant sensitising
M.J. RUIZ GÓMEZ, A. SOUVIRON, L. GIL AND M. MARTÍNEZ MORILLO
Table I. Different sensitisation levels by verapamil on human colon adenocarcinoma cells (HCA-2/1cch)
Data represent mean surviving fraction ± SD of three independent experiments in triplicate. *p<0.05;**p<0.01; ***p<0.005; ****p<0.001; (Student’s t-test).
effect for the majority of cell lines (11),
4. Goodman, G. E., Yen, Y. P., Cox, T. C., et al.
(1987): Cancer Res., 47, 2295-2304.
with our findings reported that verapamil
5. Macfarlane, D. E. and Manzel, L. (1998): J.
Immunol., 160, 1122-1131.
(10 µg/ml) did not sensitise the multidrug
6. Morales, J. A., Ruiz-Gómez, M. J., Gil-Car-
mona, L., et al. (1995): Rev. esp. Fisiol., 51, 43-
7. Pirker, R., Keilhauer, G., Raschack, M., et al.
(1990): Int. J. Cancer, 45, 916-919.
8. Ruiz Gómez, M. J., Gil, L., Souviron, A. et al.
(2000): J. Physiol. Biochem., 56, 33-38.
9. Ruiz Gómez, M. J., Pastor Vega, J. M., de la
Peña, L., et al. (1999): J. Physiol. Biochem., 55, 79-84.
10. Twentyman, P. R., Reeve, J. G., Koch, G., et al.
(1990): Br. J. Cancer, 62, 89-95.
11. Woodcock, B. G., Abdel-Rahman, M. S.,
Wosch, F., et al. (1993): Eur. J. Cancer, 29, 559- 561.
1. Bowles, A. P., Pantazis, C. G. and Wansley, W.
(1990): J. Neurosurg., 73, 248-253. Key words: Alkaloid, Colon carcinoma, Multidrug
2. Brian, L. J., Dalton, W., Fisher, G. A., et al.
resistance (MDR), P-glycoprotein, Sensitisation,
(1993): Cancer, 72, 3484-3488.
3. Cano-Gauci, D. F. and Riordan, J. R. (1987):
Biochem. Pharmacol., 36, 2115-2123. M. J. Ruiz Gómez, A. Souviron, L. Gil and M. Martínez Morillo
Correspondence to M. Martínez Morillo (Tel.: +34
Departamento de Radiología y Medicina Física,
952 131 631; Fax: +34 952 131630; e-mail: mmorillo@
Facultad de Medicina, Universidad de Málaga,
J. Physiol. Biochem., 57 (4), 2001
The Swine Flu Propaganda Explained All the fear mongering nonsense about Avian Flu/Bird flu - and now swine flu -over the past few years was to condition the population that something big wasbound to break out sooner or later. Then, when they decided the time was right -like in Mexico City - they ramped up the media / government / experts to put usThe Japanese Ministry of Health has already e
BIOLOGY 230 Review Sheets 1. Label the following as atoms, cations, anions, or molecules. ______________ Mg+2______________ H2O______________ O2______________ Na______________ Cl-______________ Ca+22. In saline (salty water), what is the solute and what is the solvent?3. Be able to recognize biomolecules indicating their major molecular group and specific type. 4. Calcium atoms have two ele