Renxiao wang, ph

Shanghai Institute of Organic Chemistry Wen Liu, Ph.D.

 Natural Product Biosynthesis (Genetics, Biochemistry and Chemistry) Interests
 Combinatorial Biosynthesis for Production Improvement and Structural  Genome Mining for Discovery of Novel Microbial Natural Products Professional
2003–present: Professor, Shanghai Institute of Organic Chemistry, Chinese
Academy of Sciences, China

2001-2003: Research Associate, School of Pharmacy, University of Wisconsin-
Madison, United States
2000–2001: Postdoctoral Fellow, Department of Chemistry, University of California,
Davis, United States

1992–1994: Teaching Associate, Department of Technology, Professional University
of Chongqing, Chongqing, China

1994-2000: M.S. & Ph.D., Chinese Academy of Medical Sciences (Peking Union
Medical College), China

 Department: Institute of Medicinal Biotechnology
 Major: Molecular Biology & Medical Biochemistry
 Director:
Prof. Yuan Li, Chinese Academy of Medical Sciences Prof. Ben Shen, University of California, Davis, United States  Thesis title: “Biosynthesis of the Enediyne Antitumor Antibiotic C-1027” 1988-1992: B.S., Sichuan University, China
 Department: Biotechnology
 Major: Microbiology
1988-1992: Student, First Middle School of Yibin, Sichuan, China
 Department: Biotechnology
 Major: Microbiology
Honors and
2013: Distinguished Lectureship Award, Chemical Society of Japan (CSJ)

Outstanding Academic Leader, Science and Technology Commission

Excellent Postgraduate Tutor, Chinese Academy of Sciences (CAS)

:Leading Talent Award, Science and Technology Commission of

Outstanding Instructor Award, Chinese Academy of Sciences (CAS)
Senauer Faye Ann Wandt Young Biology Prize, Chinese
2009: Member of Excellence of “Hundred Talents Program”, CAS
2005: Distinguished Young Scholar, Chinese National Scientific Foundation

2005: Pujiang Scholar, Science and Technology Commission of Shanghai

2005: Rising-Star Scholar, STCSM
Vice Chairman, Molecular Microbiology and Biological Engineering
Committee, Chinese Society of Microbiology
Member of Standing Committee, Enzyme engineering and Fermentation
Engineering Committee, Chinese Medicine Biological Technology

, Molecular Biology Committee, Chinese Society of Microbiology
Member, Organic Chemistry Committee, Shanghai Chemical Industry
Member, 5th Editorial Board of “Chinese Journal of Organic Chemistry”
Member, 8th Editorial Board of “Chinese Journal of Antibiotics”
Selected Recent 1. Wang, S.; Zhou, S.; Liu, W., Opportunities and Challenges from Current
Investigations into the Biosynthetic Logic of Nosiheptide-Represented
Thiopeptide Antibiotics. Current Opinion in Chemical Biology 2013, invited
2. Zhang, Q.; Pang, B.; Ding, W.; Liu, W., Aromatic polyketides produced by
bacterial Iterative type I polyketide synthases. ACS Catalysis 2013, 3, 1439-
3. Chen, D.; Zhang, L.; Pang, B.; Chen, J.; Xu, Z.; Abe, I.; Liu, W., FK506
maturation involves a cytochrome P450 protein-catalyzed four electron
oxidation in parallel with C-31 O-methylation. Journal of Bacteriology 2013,
195(9), 1931-1939.
4. Shao, L.; Chen, J.; Wang, C.; Li, J.; Tang, Y.; Chen, D.; Liu, W.,
Characterization of a key aminoglycoside phosphotransferase in gentamicin
biosynthesis. Bioorganic & Medicinal Chemistry Letters 2013, 23(5), 1438-
5. Sun, P.; Zhao, Q.; Yu, F.; Zhang, H.; Wu, Z.; Wang, Y-Y.; Wang, Y.; Liu, W.,
Spiroketal formation and modification in avermectin biosynthesis involves a
dual activity of AveC. Journal of the American Chemical Society 2012, 135
(4), 1540-1548.
6. Zhang, Q.; Liu, W., Biosynthesis of thiopeptide antibiotics and their pathway
engineering. Natural Product Reports 2013, 30, 218-226.
7. Wu, Q.; Wu, Z.; Qu, X.; Liu, W., Insights into pyrroindomycin biosynthesis
reveal a uniform paradigm for tetromate/tetronate formation. Journal of the
American Chemical Society
2012, 134, 17342-17345.
8. Duan, L.; Wang, S.; Liao, R.; Liu, W., Insights into quinaldic acid formation in
thiostrepton biosynthesis facilitating fluorinated thiopeptide generation.
Chemistry & Biology 2012, 19 (4), 443-448.
9. Qu, X.; Pang, B.; Zhang, Z.; Chen, M.; Wu, Z.; Zhao, Q.; Zhang, Q.; Wang,
Y.; Liu, Y.; Liu, W., Caerulomycins and collismycins share a common
paradigm for 2,2’-bipyridine biosynthesis via an unusual hybrid polyketide-
peptide assembly logic. Journal of the American Chemical Society 2012,
134 (22), 9038-9041.
10. Zhang, Q,; van der Donk, W. A.; Liu, W., Radical-mediated enzymatic
methylation: a tale of two SAMS. Accounts of Chemical Research 2012, 45
(4), 555-564.
11. Chen, D.; Zhang, Q.; Zhang, Q.; Cen, P.; Xu, Z.; Liu, W., Improvement of
FK506 production in Streptomyces tsukubaensis by genetic enhancement of
the supply of unusual polyketide extender untis via utilization of two distinct
site-specific recombination systems. Applied and Environmental
2012, 78 (15), 5093-5103.
12. Smanski, M. J.; Qu, X. D.; Liu, W., Shen, B. Biosynthesis of pharmaceutical
natural products and their pathway engineering. In Breakthoughs and
Perspectives in Organic Chemistry——Views Based on the
Achievements in the First Decade of the 21th Century
. Ding, K. L; Dai, L.
X. Eds. John Wiley-VCH Verlag GmbH & Co. KGaA, 2011, pp125-180.
13. Yan, Y.; Zhang, L.; Ito, T.; Qu, X.; Asakawa, Y.; Awakawa, T.; Abe, I.; Liu, W.,
Biosynthetic pathway for high structural diversity of a common dilactone core
in antimycin production. Organic Letter 2012, 14 (16), 4142-4145.
14. Li, J.; Qu, X.; He, X.; Duan, L.; Wu, G.; Bi, D.; Deng, Z.; Liu, W.*; Ou, H. Y.*,
ThioFinder: a web-based tools for the identification of thiopeptide gene
clusters in DNA sequence. PloS One 2012, 7 (9), e45878.
15. Qu, X. D.; Lei, C.; Liu, W., Transcriptome mining of active biosynthetic
pathways and their associated products in Streptomyces flaveolus.
Angewandte Chemie International Edition 2011, 50, 9651-9654.
16. Wu, J. Q.; Zhang, Q. L.; Deng, W.; Qian, J. C.; Zhang, S. L.; Liu, W., An
artificial attB site for specific recombination facilitates genetic manipulations
towards improving the erythromycin A production in an industrial
Saccharopolyspora erythraea strain. Applied and Environmental
2011, 77 (21), 7508-7516.
17. Zhang, Q.; Liu, W., Complex biotransformations catalyzed by radical S-
adenosylmethionine enzymes. Journal of Biological Chemistry 2011, 286
(35), 30245-30252.
18. Zhang, Q.; Li, Y. X.; Chen, D. D.; Yu, Y.; Duan, L. A.; Shen, B.; Liu, W.,
Radical-mediated enzymatic carbon chain fragmentation-recombination.
Nature Chemical Biology 2011, 7 (3), 154-160 (Featured in:
BIOCHEMISTRY targeting tryptophan. Science 2011, 311, 1366; Radical
break-up, blissful make-up. Nat. Chem. Biol. 2011, 7, 133-134; Chemical
biology: radical transformation. Nat. China 2011, doi: 10.1038/nchina.2011.9;
Faculty of 1000 2011, post-publication peer review).
19. Liao, R. J.; Liu, W., Thiostrepton Maturation Involving a Deesterification-
Amidation Way To Process the C-Terminally Methylated Peptide Backbone.
Journal of the American Chemical Society 2011, 133 (9), 2852-2855.
20. Qu, X. D.; Jiang, N.; Xu, F.; Shao, L.; Tang, G. L.; Wilkinson, B.; Liu, W.,
Cloning, sequencing and characterization of the biosynthetic gene cluster of sanglifehrin A, a potent cyclophilin inhibitor. Molecular Biosystems 2011, 7
(3), 852-861 (Featured in: Faculty of 1000 2011, post-publication peer
21. Zhang, Q.; Chen, D. D.; Lin, J.; Liao, R. J.; Tong, W.; Xu, Z. N.; Liu, W.,
Characterization of NocL Involved in Thiopeptide Nocathiacin I Biosynthesis A
ADENOSYLMETHIONINE ENZYME. Journal of Biological Chemistry 2011,
286 (24), 21287-21294.
22. Zhang, Q.; Wu, J.; Qian, J.; Chu, J.; Zhuang, Y.; Zhang, S.; Liu, W., Knocking
out of tailoring genes eryK and eryG in an industrial erythromycin-producing
strain of Saccharopolyspora erythraea leading to overproduction of
erythromycin B, C and D at different conversion ratios. Letters in Applied
2011, 52 (2), 129-137.
23. Ding, W.; Deng, W.; Tang, M. C.; Zhang, Q.; Tang, G. L.; Bi, Y. R.; Liu, W.,
Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into
the antitumor antibiotic azinomycin B. Molecular Biosystems 2010, 6 (6),
24. Ding, W.; Lei, C.; He, Q. L.; Zhang, Q. L.; Bi, Y. R.; Liu, W., Insights into
Bacterial 6-Methylsalicylic Acid Synthase and Its Engineering to Orsellinic
Acid Synthase for Spirotetronate Generation. Chemistry & Biology 2010, 17
(5), 495-503.
25. Ding, Y.; Yu, Y.; Pan, H. X.; Guo, H.; Li, Y. M.; Liu, W., Moving
posttranslational modifications forward to biosynthesize the glycosylated
thiopeptide nocathiacin I in Nocardia sp ATCC202099. Molecular
2010, 6 (7), 1180-1185.
26. Wang, J.; Yu, Y.; Tang, K. X.; Liu, W.; He, X. Y.; Huang, X.; Deng, Z. X.,
Identification and Analysis of the Biosynthetic Gene Cluster Encoding the
Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-
22. Applied and Environmental Microbiology 2010, 76 (7), 2335-2344.
27. Yu, Y.; Guo, H.; Zhang, Q.; Duan, L. A.; Ding, Y.; Liao, R. J.; Lei, C.; Shen,
B.; Liu, W., NosA Catalyzing Carboxyl-Terminal Amide Formation in
Nosiheptide Maturation via an Enamine Dealkylation on the Serine-Extended
Precursor Peptide. Journal of the American Chemical Society 2010, 132
(46), 16324-16326.
28. He, Q. L.; Jia, X. Y.; Tang, M. C.; Tian, Z. H.; Tang, G.; Liu, W., Dissection of
Two Acyl-Transfer Reactions Centered on Acyl-S-Carrier Protein
Intermediates for Incorporating 5-Chloro-6-methyl-O-methylsalicyclic Acid into
Chlorothricin. ChemBioChem 2009, 10 (5), 813-819.
29. Liao, R. J.; Duan, L.; Lei, C.; Pan, H. X.; Ding, Y.; Zhang, Q.; Chen, D. J.;
Shen, B.; Yu, Y.; Liu, W., Thiopeptide Biosynthesis Featuring Ribosomally
Synthesized Precursor Peptides and Conserved Posttranslational
Modifications. Chemistry & Biology 2009, 16 (2), 141-147 (Featured in: This
week in techniques: Biosynthetic pathway for the production of thiopeptide
antibiotics. SciBX 2(10), 2009, Doi: 10.1038/scibx.2009.423; Thiopeptide
antibiotic biosynthesis. Angew. Chem. Int. Ed. 2009, 48,6770-6773; Recent
advances in thiopeptide antibiotic biosynthesis. Nat. Prod. Rep. 2010, 27,
30. Yu, Y.; Duan, L.; Zhang, Q.; Liao, R. J.; Ding, Y.; Pan, H. X.; Wendt-
Pienkowski, E.; Tang, G. L.; Shen, B.; Liu, W., Nosiheptide Biosynthesis
Featuring a Unique Indole Side Ring Formation on the Characteristic
Thiopeptide Framework. ACS Chemical Biology 2009, 4 (10), 855-864
(Featured in: Recent advances in thiopeptide antibiotic biosynthesis. Nat.
Prod. Rep.
2010, 27, 153-164).
31. Liu, W.; Yu, Y, Combinatorial Biosynthesis of pharmaceutical natural
products. Biocatalysis for the pharmaceutical industry-discovery, development
and manufacturing. In Biocatalysis for the Pharmaceutical Industry. Tao,
J. H.; Lin, G. Q.; Liese, A. Eds. John Wiley & Sons Pte Ltd, 2009, pp229-245.
32. Chen, Y.; Deng, W.; Wu, J. Q.; Qian, J. C.; Chu, J.; Zhuang, Y. P.; Zhang, S.
L.; Liu, W., Genetic modulation of the overexpression of tailoring genes eryK
and eryG leading to the improvement of erythromycin a purity and production
in Saccharopolyspora erythraea fermentation. Applied and Environmental
2008, 74 (6), 1820-1828.
33. Fang, J.; Zhang, Y. P.; Huang, L. J.; Jia, X. Y.; Zhang, Q.; Zhang, X.; Tang,
G.; Liu, W., Cloning and characterization of the tetrocarcin a gene cluster
from Micromonospora chalcea NRRL 11289 reveals a highly conserved
strategy for tetronate biosynthesis in spirotetronate antibiotics. Journal of
2008, 190 (17), 6014-6025.
34. Galm, U.; Wang, L. Y.; Wendt-Pienkowski, E.; Yang, R. Y.; Liu, W.; Tao, M.
F.; Coughlin, J. M.; Shen, B., In vivo manipulation of the bleomycin
biosynthetic gene cluster in Streptomyces verticillus ATCC15003 revealing
new insights into its biosynthetic pathway. Journal of Biological Chemistry
2008, 283 (42), 28236-28245.
35. Li, L.; Deng, W.; Song, J.; Ding, W.; Zhao, Q. F.; Peng, C.; Song, W. W.;
Tang, G.; Liu, W., Characterization of the saframycin a gene cluster from
Streptomyces lavendulae NRRL 11002 revealing a nonribosomal peptide
synthetase system for assembling the unusual tetrapeptidyl skeleton in an
iterative manner. Journal of Bacteriology 2008, 190 (1), 251-263.
36. Zhang, J.; Van Lanen, S. G.; Ju, J. H.; Liu, W.; Dorrestein, P. C.; Li, W. L.;
Kelleher, N. L.; Shen, B., A phosphopantetheinylating polyketide synthase
producing a linear polyene to initiate enediyne antitumor antibiotic
biosynthesis. Proceedings of the National Academy of Sciences of the
United States of America
2008, 105 (5), 1460-1465 (Featured in:
Enediynes taught how to work better. C&E News 2007, 85, 25).
37. Zhao, Q. F.; He, Q. L.; Ding, W.; Tang, M. C.; Kang, Q. J.; Yu, Y.; Deng, W.;
Zhang, Q.; Fang, J.; Tang, G.; Liu, W., Characterization of the azinomycin B
biosynthetic gene cluster revealing a different iterative type I polyketide
synthase for naphthoate biosynthesis. Chemistry & Biology 2008, 15 (7),
693-705 (Featured in: Antibiotic biosynthesis: from Genes to Enzymes. Nat.
2008, Doi: 10.1038/nchina.2008.196).
38. Liu, W.; Zhang, S. L.; Chen, Y.; Deng, W.; Wu, J. Q., Improvement of
Erythromycin a purity and production at the fermentation stage of
Saccharopolyspora erytharea by metabolic engineering. Journal of
2008, 136 (Supplement 1): S21-S21.
39. Kennedy, D. R.; Gawron, L. S.; Ju, J. H.; Liu, W.; Shen, B.; Beerman, T. A.,
Single chemical modifications of the C-1027 enediyne core, a radiomimetic
antitumor drug, affect both drug potency and the role of ataxia-telangiectasia
mutated in cellular responses to DNA double-strand breaks. Cancer
2007, 67 (2), 773-781.
40. Shen, B.; Cheng, Y. Q.; Christenson, S. D.; Jiangi, H.; Ju, J. H.; Kwon, H. J.;
Lim, S. K.; Liu, W.; Nonaka, K.; Seo, J. W.; Smith, W. C.; Standage, S.; Tang,
G. L.; Van Lanen, S.; Zhang, J., Polyketide Biosynthesis beyond the Type I, II,
and III Polyketide Synthase Paradigms: A Progress Report. In Polyketides:
Biosynthesis, Biological Activity, and Genetic Engineering
, Rimando, A.
M.; Baerson, S. R., Eds. 2007; Vol. 955, pp 154-166.
41. Van Lanen, S. G.; Oh, T. J.; Liu, W.; Wendt-Pienkowski, E.; Shen, B.,
Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. Journal of the American Chemical Society 2007,
129 (43), 13082-13094.
42. Jia, X. Y.; Tian, Z. H.; Shao, L.; Qu, X. D.; Zhao, Q. F.; Tang, J.; Tang, G.;
Liu, W., Genetic characterization of the chlorothricin gene cluster as a model
for spirotetronate antibiotic biosynthesis. Chemistry & Biology 2006, 13 (6),
43. Shao, L.; Qu, X. D.; Jia, X. Y.; Zhao, Q. F.; Tian, Z. H.; Wang, M.; Tang, G.;
Liu, W., Cloning and characterization of a bacterial iterative type I polyketide
synthase gene encoding the 6-methylsalicyclic acid synthase. Biochemical
and Biophysical Research Communications
2006, 345 (1), 133-139.
44. Liu, W.; Nonaka, K.; Nie, L. P.; Zhang, J.; Christenson, S. D.; Bae, J.; Van
Lanen, S. G.; Zazopoulos, E.; Farnet, C. M.; Yang, C. F.; Shen, B., The
neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus
ATCC 15944 involving two iterative type I polyketide synthases. Chemistry &
2005, 12 (3), 293-302.
45. Van Lanen, S. G.; Dorrestein, P. C.; Christenson, S. D.; Liu, W.; Ju, J. H.;
Kelleher, N. L.; Shen, B., Biosynthesis of the beta-amino acid moiety of the
enediyne antitumor antibiotic C-1027 featuring beta-amino acyl-S-carrier
protein intermediates. Journal of the American Chemical Society 2005,
127 (33), 11594-11595.
46. Murrell, J. M.; Liu, W.; Shen, B., Biochemical characterization of the SgcA1
alpha-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne
antitumor antibiotic C-1027 biosynthetic pathway and overexpression of
sgcA1 in Streptomyces globisporus to improve C-1027 production. Journal of
Natural Products
2004, 67 (2), 206-213.
47. Christenson, S. D.; Liu, W.; Toney, M. D.; Shen, B., A novel 4-
methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne
antitumor antibiotic C-1027 biosynthesis. Journal of the American Chemical
2003, 125 (20), 6062-6063.
48. Liu, W.; Ahlert, J.; Gao, Q. J.; Wendt-Pienkowski, E.; Shen, B.; Thorson, J.
S., Rapid PCR amplification of minimal enediyne polyketide synthase
cassettes leads to a predictive familial classification model. Proceedings of
the National Academy of Sciences of the United States of America
100 (21), 11959-11963.
49. Shen, B.; Liu, W.; Nonaka, K., Enediyne natural products: Biosynthesis and
prospect towards engineering novel antitumor agents. Current Medicinal
2003, 10 (21), 2317-2325.
50. Zazopoulos, E.; Huang, K. X.; Staffa, A.; Liu, W.; Bachmann, B. O.; Nonaka,
K.; Ahlert, J.; Thorson, J. S.; Shen, B.; Farnet, C. M., A genomics-guided
approach for discovering and expressing cryptic metabolic pathways. Nature
2003, 21 (2), 187-190.
51. Liu, W.; Christenson, S. D.; Standage, S.; Shen, B., Biosynthesis of the
enediyne antitumor antibiotic C-1027. Science 2002, 297 (5584), 1170-1173
(featured in: New enzymes in warhead. Nat. Biotechnol. 2002, 20, 984-985;
Towards bioengineering anticancer drugs. Chem. & Biol. 2002, 9, 956-958;
Newly mapped bacterial genes may contain blueprints for new anticancer
drugs. The lancet, 2002, 360, 550; Path to enediynes: Engineering of
biosynthetic route could lead to better anticancer drugs. C&E News, 2002,
80, 33; Chemical Highlights 2002: Biochemistry. C&E News, 2002, 80, 40).
52. Liu, W.; Shen, B., Genes for production of the enediyne antitumor antibiotic
C-1027 in Streptomyces globisporus are clustered with the cagA gene that
encodes the C-1027 apoprotein. Antimicrobial Agents and Chemotherapy
2000, 44 (2), 382-392 (Featured in: Enediyne research continues apace.
C&E News, 2000, 78, 47-49).
53. Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J., Enediyne
biosynthesis and self-resistance: A progress report. Bioorganic Chemistry
1999, 27 (2), 172-188.


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